Author(s): Jose S, Fangueiro JF, Smitha J, Cinu TA, Chacko AJ, et al.
Insulin-loaded chitosan microspheres were engineered by emulsion cross-linking method using glutaraldehyde as cross-linker. Taguchi orthogonal method was applied to optimize the production time and reduce the number of experiments required to obtain an optimized formulation. Three variables were evaluated, i.e. chitosan and glutaraldehyde concentrations, and cross-linking time at three levels. The dependent variables were the mean particle size and the encapsulation efficiency. The optimal formulation was obtained with chitosan 3% (w/v), glutaraldehyde 3.5% (v/v), and cross-linking time of 5h, characterized by microspheres with a mean particle size of 29.5 μm, and insulin encapsulation efficiency of 71.6±1.3%. In vivo studies were carried out using male Wistar albino rats, revealing a significant reduction in blood glucose level after administration of the optimized formulation, in comparison to a subcutaneous insulin injection. Chitosan microspheres were superior in terms of sustaining protein release over conventional insulin therapy.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/22221459
Author(s): Beals J, Brader M, De Felippis M, Kovach P
Author(s): American Diabetes Association
Author(s): Fonte P, Araújo F, Reis S, Sarmento B
Author(s): Rosenfeld L
Author(s): Al-Tabakha MM, Arida AL
Author(s): Bhatia E, Aggarwal A
Author(s): Chaturvedi K, Ganguly K, Nadagouda MN, Aminabhavi TM
Author(s): Magwire ML
Author(s): Arbit E, Kidron M
Author(s): Varshosaz J
Author(s): Heinemann L, Jacques Y
Author(s): Eldor R, Arbit E, Schurr D, Kidron M, Hersko A
Author(s): Gu Z, Aimetti AA, Wang Q, Dang TT, Zhang Y, et al.
Author(s): Trapani A, Lopedota A, Franco M, Cioffi N, Ieva E, et al.
Author(s): Cui F, Qian F, Zhao Z, Yin L, Tang C, et al.
Author(s): Li T, Shi XW, Du YM, Tang YF
Author(s): Pan Y, Li YJ, Zhao HY, Zheng JM, Xu H, et al.
Author(s): Ma Z, Yeoh HH, Lim LY
Author(s): Zhang N, Ping Q, Huang G, Xu W, Cheng Y, et al.
Author(s): Chen MC, Sonaje K, Chen KJ, Sung HW
Author(s): Babu VR, Patel P, Mundargi RC, Rangaswamy V, Aminabhavi TM
Author(s): Damgé C, Reis CP, Maincent P
Author(s): des Rieux A, Fievez V, Garinot M, Schneider YJ, Préat V
Author(s): Michel C, Aprahamian M, Defontaine L, Couvreur P, Damgé C
Author(s): Damgé C, Michel C, Aprahamian M, Couvreur P
Author(s): Sajeesh S, Sharma CP
Author(s): Carino GP, Jacob JS, Mathiowitz E
Author(s): Chaudhury A, Das S
Author(s): Niu M, Lua Y, Hovgaard L, Guan P, Tan Y, et al.
Author(s): Xiong XY, Li YP, Li ZL, Zhou CL, Tam KC, et al.
Author(s): Sarmento B, Martins S, Ferreira D, Souto EB
Author(s): Elsayed AM
Author(s): Sung HW, Sonaje K, Feng SS
Author(s): Chalasani KB, Russell-Jones GJ, Jain AK, Diwan PV, Jain SK
Author(s): Severino P, Andreani T, Macedo AS, Fangueiro JF, Santana MH, et al.
Author(s): Ramachandran R, Paul W, Sharma CP
Author(s): Mundargi RC, Rangaswamy V, Aminabhavi TM
Author(s): Mundargi RC, Rangaswamy V, Aminabhavi TM
Author(s): Najafzadeh H, Kooshapur H, Kianidehkordi F
Author(s): Sung HW, Sonaje K, Liao ZX, Hsu LW, Chuang EY
Author(s): Pridgen EM, Alexis F, Kuo TT, Levy-Nissenbaum E, Karnik R, et al.
Author(s): Sabetsky V, Ekblom J
Author(s): Castor TP