Author(s): Zhang H, DiBaise JK, Zuccolo A, Kudrna D, Braidotti M, et al.
Recent evidence suggests that the microbial community in the human intestine may play an important role in the pathogenesis of obesity. We examined 184,094 sequences of microbial 16S rRNA genes from PCR amplicons by using the 454 pyrosequencing technology to compare the microbial community structures of 9 individuals, 3 in each of the categories of normal weight, morbidly obese, and post-gastric-bypass surgery. Phylogenetic analysis demonstrated that although the Bacteria in the human intestinal community were highly diverse, they fell mainly into 6 bacterial divisions that had distinct differences in the 3 study groups. Specifically, Firmicutes were dominant in normal-weight and obese individuals but significantly decreased in post-gastric-bypass individuals, who had a proportional increase of Gammaproteobacteria. Numbers of the H(2)-producing Prevotellaceae were highly enriched in the obese individuals. Unlike the highly diverse Bacteria, the Archaea comprised mainly members of the order Methanobacteriales, which are H(2)-oxidizing methanogens. Using real-time PCR, we detected significantly higher numbers of H(2)-utilizing methanogenic Archaea in obese individuals than in normal-weight or post-gastric-bypass individuals. The coexistence of H(2)-producing bacteria with relatively high numbers of H(2)-utilizing methanogenic Archaea in the gastrointestinal tract of obese individuals leads to the hypothesis that interspecies H(2) transfer between bacterial and archaeal species is an important mechanism for increasing energy uptake by the human large intestine in obese persons. The large bacterial population shift seen in the post-gastric-bypass individuals may reflect the double impact of the gut alteration caused by the surgical procedure and the consequent changes in food ingestion and digestion.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/19164560
Author(s): Arab JP, Candia R, Zapata R, Muñoz C, Arancibia JP, et al.
Author(s): Machado MV, Cortez-Pinto H1
Author(s): Aron-Wisnewsky J, Gaborit B, Dutour A, Clement K
Author(s): Lambert JE, Ramos-Roman MA2, Browning JD3, Parks EJ4
Author(s): Donnelly KL, Smith CI, Schwarzenberg SJ, Jessurun J, Boldt MD, et al.
Author(s): Furukawa S, Fujita T, Shimabukuro M, Iwaki M, Yamada Y, et al.
Author(s): Tateya S, Kim F, Tamori Y
Author(s): Gangarapu V, Yıldız K, Ince AT, Baysal B
Author(s): Miura K, Ohnishi H1
Author(s): Machado M, Marques-Vidal P, Cortez-Pinto H
Author(s): Anstee QM, Targher G, Day CP
Author(s): Targher G, Bertolini L, Padovani R, Rodella S, Tessari R, et al.
Author(s): Goel A, Gupta M, Aggarwal R
Author(s): Turnbaugh PJ, Ley RE, Mahowald MA, Magrini V, Mardis ER, et al.
Author(s): Ley RE, Bäckhed F, Turnbaugh P, Lozupone CA, Knight RD, et al.
Author(s): Musso G, Gambino R, Cassader M
Author(s): Tilg H, Kaser A
Author(s): Ley RE, Turnbaugh PJ, Klein S, Gordon JI
Author(s): Schwiertz A, Taras D, Schäfer K, Beijer S, Bos NA, et al.
Author(s): Duncan SH, Lobley GE, Holtrop G, Ince J, Johnstone AM, et al.
Author(s): Arumugam M, Raes J, Pelletier E, Le Paslier D, Yamada T, et al.
Author(s): Bäckhed F, Ding H, Wang T, Hooper LV, Koh GY, et al.
Author(s): Bäckhed F, Manchester JK, Semenkovich CF, Gordon JI
Author(s): Parekh PJ, Arusi E, Vinik AI2, Johnson DA3
Author(s): Shen J, Obin MS, Zhao L
Author(s): Lin HV, Frassetto A, Kowalik EJ Jr, Nawrocki AR, Lu MM, et al.
Author(s): Caricilli AM, Picardi PK, de Abreu LL, Ueno M, Prada PO, et al.
Author(s): Spor A, Koren O, Ley R
Author(s): Caricilli AM, Saad MJ
Author(s): Tsukumo DM, Carvalho-Filho MA, Carvalheira JB, Prada PO, Hirabara SM, et al.
Author(s): Zhao L, Hu P, Zhou Y, Purohit J, Hwang D
Author(s): Tamrakar AK, Schertzer JD, Chiu TT, Foley KP, Bilan PJ, et al.
Author(s): Winder WW, Hardie DG
Author(s): Tilg H, Moschen AR
Author(s): Franchi L, Eigenbrod T, Muñoz-Planillo R, Nuñez G
Author(s): Kim KA, Gu W, Lee IA, Joh EH, Kim DH
Author(s): Rivera CA, Adegboyega P, van Rooijen N, Tagalicud A, Allman M, et al.
Author(s): Takaki A, Kawai D, Yamamoto K
Author(s): Ye D, Li FY, Lam KS, Li H, Jia W, et al.
Author(s): Miura K, Kodama Y, Inokuchi S, Schnabl B, Aoyama T, et al.
Author(s): Ehses JA, Meier DT, Wueest S, Rytka J, Boller S, et al.
Author(s): Himes RW, Smith CW
Author(s): Rivera CA, Gaskin L, Allman M, Pang J, Brady K, et al.
Author(s): Szabo G, Velayudham A, Romics L Jr, Mandrekar P
Author(s): Cario E, Gerken G, Podolsky DK
Author(s): Vijay-Kumar M, Aitken JD, Carvalho FA, Cullender TC, Mwangi S, et al.
Author(s): Elinav E, Strowig T, Kau AL, Henao-Mejia J, Thaiss CA, et al.
Author(s): Chen GY, Liu M, Wang F, Bertin J, Núñez G
Author(s): Henao-Mejia J, Elinav E, Jin C, Hao L, Mehal WZ, et al.
Author(s): Wlodarska M, Thaiss CA2, Nowarski R3, Henao-Mejia J3, Zhang JP4, et al.
Author(s): Anand PK, Malireddi RK, Lukens JR, Vogel P, Bertin J, et al.
Author(s): Kamari Y, Shaish A, Vax E, Shemesh S, Kandel-Kfir M, et al.
Author(s): Cani PD, Bibiloni R, Knauf C, Waget A, Neyrinck AM, et al.
Author(s): Bergheim I, Weber S, Vos M, Krämer S, Volynets V, et al.
Author(s): Gibson GR, Roberfroid MB
Author(s): Gibson GR, Probert HM, Loo JV, Rastall RA, Roberfroid MB
Author(s): Slavin J1
Author(s): Parnell JA, Raman M, Rioux KP, Reimer RA
Author(s): Macfarlane S, Macfarlane GT, Cummings JH
Author(s): Parnell JA, Reimer RA
Author(s): Daubioul C, Rousseau N, Demeure R, Gallez B, Taper H, et al.
Author(s): Levrat MA, Rémésy C, Demigné C
Author(s): Topping DL, Clifton PM
Author(s): Cani PD, Neyrinck AM, Fava F, Knauf C, Burcelin RG, et al.
Author(s): Cani PD, Possemiers S, Van de Wiele T, Guiot Y, Everard A, et al.
Author(s): Beylot M1
Author(s): Parnell JA, Reimer RA
Author(s): Sanders ME1
Author(s): Iacono A, Raso GM, Canani RB, Calignano A, Meli R
Author(s): Ma YY, Li L, Yu CH, Shen Z, Chen LH, et al.