Author(s): Westgate EJ, Cheng Y, Reilly DF, Price TS, Walisser JA, et al.
Background: Myocardial infarction, stroke, and sudden death undergo diurnal variation. Although genes relevant to hemostasis and vascular integrity undergo circadian oscillation, the role of the molecular clock in thrombotic events remains to be established.
Methods and results: A diurnal variation in the time to thrombotic vascular occlusion (TTVO) subsequent to a photochemical injury was observed in wild-type mice: TTVO varied from 24.6+/-2.7 minutes at zeitgeber time (ZT) 2 to 40.3+/-4.3 minutes at ZT8, 24.3+/-2.3 minutes at ZT14, and 31.0+/-4.4 minutes at ZT20. This pattern was disrupted or altered when core clock genes-BMAL1, CLOCK, and NPAS2-were mutated or deleted. Mutation of CLOCK abolished the diurnal variation in TTVO, whereas deletion of NPAS2 altered its temporal pattern. NPAS2 deletion prolonged TTVO and reduced blood pressure irrespective of clock time. Global BMAL1 deletion shortened TTVO at ZT8, and the diurnal variation in TTVO, but not in systemic blood pressure, was disrupted in mice in which BMAL1 had been selectively deleted in endothelium.
Conclusions: Key components of the molecular clock regulate the response to a thrombogenic stimulus in vivo. Such a phenomenon may interact with environmental variables, and together with the influence of these genes on blood pressure may contribute to the diurnal variation in cardiovascular events observed in humans.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/18413500
Author(s): Best JD, Maywood ES, Smith KL, Hastings MH
Author(s): Stokkan KA, Yamazaki S, Tei H, Sakaki Y, Menaker M
Author(s): Bargiello TA, Jackson FR, Young MW
Author(s): Shearman LP, Sriram S, Weaver DR, Maywood ES, Chaves I, et al.
Author(s): Panda S, Antoch MP, Miller BH, Su AI, Schook AB, et al.
Author(s): Muller JE, Tofler GH, Stone PH
Author(s): Tsementzis SA, Gill JS, Hitchcock ER, Gill SK, Beevers DG
Author(s): Lavery CE, Mittleman MA, Cohen MC, Muller JE, Verrier RL
Author(s): Manfredini R, Boari B, Smolensky MH, Salmi R, la Cecilia O, et al.
Author(s): Anea CB, Zhang M, Stepp DW, Simkins GB, Reed G, et al.
Author(s): Rudic RD, Fulton DJ
Author(s): Marshall J
Author(s): Elliott WJ
Author(s): Pardiwalla FK, Yeolekar ME, Bakshi SK
Author(s): Turin TC, Kita Y, Rumana N, Nakamura Y, Takashima N, et al.
Author(s): Casetta I, Granieri E, Fallica E, la Cecilia O, Paolino E, et al.
Author(s): Argentino C, Toni D, Rasura M, Violi F, Sacchetti ML, et al.
Author(s): Marsh EE 3rd, Biller J, Adams HP Jr, Marler JR, Hulbert JR, et al.
Author(s): Chaturvedi S, Adams HP Jr, Woolson RF
Author(s): Marler JR, Price TR, Clark GL, Muller JE, Robertson T, et al.
Author(s): Inagawa T
Author(s): Nagakane Y, Miyashita K, Nagatsuka K, Yamawaki T, Naritomi H
Author(s): Guan JW, Chen MJ, Li H, Liu YY, You C, et al.
Author(s): Guan J, Ding Y, Liu Y, Li Y, Liu Y, et al.
Author(s): Feigin VL, Anderson CS, Rodgers A, Bennett DA
Author(s): Miranpuri AS, Aktüre E, Baggott CD, Miranpuri A, Uluç K, et al.
Author(s): Temes RE, Bleck T, Dugar S, Ouyang B, Mohammad Y, et al.
Author(s): Kelly-Hayes M, Wolf PA, Kase CS, Brand FN, McGuirk JM, et al.
Author(s): Nyquist PA, Brown RD Jr, Wiebers DO, Crowson CS, O'Fallon WM
Author(s): Inagawa T, Takechi A, Yahara K, Saito J, Moritake K, et al.
Author(s): Passero S, Reale F, Ciacci G, Zei E
Author(s): Abe T, Ohde S, Ishimatsu S, Ogata H, Hasegawa T, et al.
Author(s): Inagawa T
Author(s): Brezinski DA, Tofler GH, Muller JE, Pohjola-Sintonen S, Willich SN, et al.
Author(s): Tofler GH, Brezinski D, Schafer AI, Czeisler CA, Rutherford JD, et al.
Author(s): Panza JA, Epstein SE, Quyyumi AA
Author(s): Jain S, Namboodri KK, Kumari S, Prabhakar S
Author(s): Castilla-Guerra L, Espino-Montoro A, Fernandez-Moreno MC, Lopez-Chozas JM
Author(s): Naess H, Idicula T, Brogger J, Waje-Andreassen U, Thomassen L
Author(s): Spengos K, Vemmos K, Tsivgoulis G, Manios E, Zakopoulos N, et al.
Author(s): Li-Saw-Hee FL, Blann AD, Lip GY
Author(s): Riccio PM, Klein FR, PaganiCassará F, Muñoz Giacomelli F, González Toledo ME, et al.
Author(s): Takahashi JS, Hong HK, Ko CH, McDearmon EL
Author(s): Yagita K, Tamanini F, Yasuda M, Hoeijmakers JH, van der Horst GT, et al.
Author(s): Meng QJ, Logunova L, Maywood ES, Gallego M, Lebiecki J, et al.
Author(s): Preitner N, Damiola F, Lopez-Molina L, Zakany J, Duboule D, et al.