Author(s): Wark PA, Johnston SL, Bucchieri F, Powell R, Puddicombe S, et al.
Rhinoviruses are the major trigger of acute asthma exacerbations and asthmatic subjects are more susceptible to these infections. To investigate the underlying mechanisms of this increased susceptibility, we examined virus replication and innate responses to rhinovirus (RV)-16 infection of primary bronchial epithelial cells from asthmatic and healthy control subjects. Viral RNA expression and late virus release into supernatant was increased 50- and 7-fold, respectively in asthmatic cells compared with healthy controls. Virus infection induced late cell lysis in asthmatic cells but not in normal cells. Examination of the early cellular response to infection revealed impairment of virus induced caspase 3/7 activity and of apoptotic responses in the asthmatic cultures. Inhibition of apoptosis in normal cultures resulted in enhanced viral yield, comparable to that seen in infected asthmatic cultures. Examination of early innate immune responses revealed profound impairment of virus-induced interferon-beta mRNA expression in asthmatic cultures and they produced >2.5 times less interferon-beta protein. In infected asthmatic cells, exogenous interferon-beta induced apoptosis and reduced virus replication, demonstrating a causal link between deficient interferon-beta, impaired apoptosis and increased virus replication. These data suggest a novel use for type I interferons in the treatment or prevention of virus-induced asthma exacerbations.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/15781584
Author(s): Robinson KA, Baughman W, Rothrock G, Barrett NL, Pass M, et al.
Author(s): Talbot TR, Hartert TV, Mitchel E, Halasa NB, Arbogast PG, et al.
Author(s): Juhn YJ, Kita H, Yawn BP, Boyce TG, Yoo KH, et al.
Author(s): Beard CM, Yunginger JW, Reed CE, O'Connell EJ, Silverstein MD
Author(s): Hunt LW Jr, Silverstein MD, Reed CE, O'Connell EJ, O'Fallon WM, et al.
Author(s): Bauer BA, Reed CE, Yunginger JW, Wollan PC, Silverstein MD
Author(s): Silverstein MD, Yunginger JW, Reed CE, Petterson T, Zimmerman D, et al.
Author(s): Juhn YJ, Sauver JS, Katusic S, Vargas D, Weaver A, et al.
Author(s): Juhn YJ, Weaver A, Katusic S, Yunginger J
Author(s): Juhn YJ, Kita H, Lee LA, Smith RW, Bagniewski SM, et al.
Author(s): Yawn BP, Yunginger JW, Wollan PC, Reed CE, Silverstein MD, et al.
Author(s): Juhn YJ, Kita H, Lee LA, Swanson RJ, Smith R, et al.
Author(s): Laurichesse H, Romaszko JP, Nguyen LT, Souweine B, Poirier V, et al.
Author(s): Smart JM, Kemp AS
Author(s): Fahy JV, Corry DB, Boushey HA
Author(s): Contoli M, Message SD, Laza-Stanca V, Edwards MR, Wark PA, et al.
Author(s): Grove DI, Burston TO, Wellby ML, Ford RM, Forbes IJ
Author(s): Lee HJ, Kang JH, Henrichsen J, Konradsen HB, Jang SH, et al.
Author(s): Lahood N, Emerson SS, Kumar P, Sorensen RU
Author(s): Fischer JE, Johnson JE, Kuli-Zade RK, Johnson TR, Aung S, et al.
Author(s): Kincy-Cain T, Clements JD, Bost KL
Author(s): Jung JA, Kita H, Dhillon R, Jacobson RM, Nahm MH, et al.
Author(s): Khan AQ, Shen Y, Wu ZQ, Wynn TA, Snapper CM
Author(s): Iwasaka H, Noguchi T
Author(s): Muret J, Marie C, Fitting C, Payen D, Cavaillon JM