Author(s): Lama VN , Smith L, Badri L, Flint A, Andrei AC, et al.
The origin and turnover of connective tissue cells in adult human organs, including the lung, are not well understood. Here, studies of cells derived from human lung allografts demonstrate the presence of a multipotent mesenchymal cell population, which is locally resident in the human adult lung and has extended life span in vivo. Examination of plastic-adherent cell populations in bronchoalveolar lavage samples obtained from 76 human lung transplant recipients revealed clonal proliferation of fibroblast-like cells in 62% (106 of 172) of samples. Immunophenotyping of these isolated cells demonstrated expression of vimentin and prolyl-4-hydroxylase, indicating a mesenchymal phenotype. Multiparametric flow cytometric analyses revealed expression of cell-surface proteins, CD73, CD90, and CD105, commonly found on mesenchymal stem cells (MSCs). Hematopoietic lineage markers CD14, CD34, and CD45 were absent. Multipotency of these cells was demonstrated by their capacity to differentiate into adipocytes, chondrocytes, and osteocytes. Cytogenetic analysis of cells from 7 sex-mismatched lung transplant recipients harvested up to 11 years after transplant revealed that 97.2% +/- 2.1% expressed the sex genotype of the donor. The presence of MSCs of donor sex identity in lung allografts even years after transplantation provides what we believe to be the first evidence for connective tissue cell progenitors that reside locally within a postnatal, nonhematopoietic organ.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/17347686
Author(s): Abbott OA, Hopkins WA, Van Fleit WE , Robinson JS
Author(s): Barnes PJ
Author(s): Barnes PJ , Shapiro SD, Pauwels RA
Author(s): Barnes PJ
Author(s): Bast A , Haenen GR, Doelman CJ
Author(s): Bianco P, Robey P G
Author(s): BittmannI , Dose T, Baretton GB, Müller C, Schwaiblmair M, et al.
Author(s): Böyum A
Author(s): Brantigan O C, Mueller E, Kress M B
Author(s): Cooper JD, Patterson GA, Grossman R, Maurer J
Author(s): Cooper JD, Trulock EP, Triantafillou AN, Patterson GA, Pohl MS, et al.
Author(s): Dallegri F, Ottonello L
Author(s): Di Stefano A, Maestrelli P, Roggeri A, Turato G, Calabro S, et al.
Author(s): Di Stefano A, Capelli A, Lusuardi M, Balbo P, Vecchio C, et al.
Author(s): Eriksson S
Author(s): Holden C, Vogel G
Author(s): Huang Q, Liu D, Majewski P, Schulte LC, Korn JM, et al.
Author(s): Hyatt RE1, Farkas G, Schroeder M
Author(s): Ishizawa K, Kubo H, Yamada M, Kobayashi S, Numasaki M et al.
Author(s): Koca E, Champlin RE
Author(s): Kotton DN, Ma BY, Cardoso WV, Sanderson EA, Summer RS, et al.
Author(s): Hogg J C, Walker B A
Author(s): Nikula KJ , March TH, Seagrave J, Finch G, Barr E, et al.
Author(s): Orlic D, Kajstura J, Chimenti S, Limana F, Jakoniuk I, et al.
Author(s): Prockop DJ
Author(s): Lagasse E , Connors H, Al-Dhalimy M, Reitsma M, Dohse M, et al.
Author(s): Krause DS, Theise ND, Collector MI, Henegariu O, Hwang S, et al.
Author(s): Finkelstein R, Fraser RS, Ghezzo H, Cosio MG
Author(s): Hellermann G R, Nagy S B, Kong, X.; Lockey, R.F.Mohapatra, S.S.
Author(s): Lopez AD, Murray CC
Author(s): Mahadeva R , Shapiro SD
Author(s): Martin GR
Author(s): Pushpakon R, Hogg J C, Woolcock A J, Angus A E, Macklem P T et al.
Author(s): Retamales I , Elliott WM, Meshi B, Coxson HO, Pare PD, et al.
Author(s): Russell RE , Thorley A, Culpitt SV, Dodd S, Donnelly LE, et al.
Author(s): Gordon B
Author(s): Gross P, Pfitzer E A, Tolker E, Babyak M A, Kaschak M
Author(s): Saetta M, Di Stefano A, Turato G, Facchini FM, Corbino L, et al.
Author(s): Saetta M, Turato G, Maestrelli P, Mapp CE, Fabbri LM
Author(s): Schrepfer S, Deuse T, Reichenspurner H, Fischbein MP, Robbins RC, et al.
Author(s): Senior R M, Anthonisen N R
Author(s): Shapiro SD
Author(s): Suratt BT, Cool CD, Serls AE, Chen L, Varella-Garcia M, et al.
Author(s): Takubo Y, Guerassimov A, Ghezzo H, Triantafillopoulos A, Bates JH, et al.
Author(s): Till JE, McCulloch EA
Author(s): Turhan AG
Author(s): Van Bekkum DW
Author(s): Knudson R J, Gaensler E A
Author(s): Wagers AJ, Weissman IL
Author(s): Wagner J G, Roth RA
Author(s): Yamada M, Kubo H, Kobayashi S, Ishizawa K, Numasaki M, et al.
Author(s): Xu J, Qu J, Cao L, Sai Y, Chen C, et al.
Author(s): Carrell RW
Author(s): Vidal R, Blanco I, Casas F, Jardí R, Miravitlles M; Committee on the National Registry of Individuals with Alpha-1 Antitrypsin Deficiency
Author(s): Costa X, Jardi R, Rodriguez F, Miravitlles M, Cotrina M, et al.
Author(s): Stoller JK, Aboussouan LS
Author(s): Seixas S, Garcia O, Trovoada MJ, Santos MT, Amorim A, et al.
Author(s): de Serres FJ
Author(s): Menezes AM, Perez-Padilla R, Jardim JR, Muiño A, Lopez MV, et al.
Author(s): de Faria EJ, de Faria IC, Alvarez AE, Ribeiro JD, Ribeiro AF, et al.
Author(s): Cruz PE, Mueller C, Cossette TL, Golant A, Tang Q, et al.
Author(s): Stockley RA, Luisetti M, Miravitlles M, Piitulainen E, Fernandez P; Alpha One International Registry (AIR) group
Author(s): Martorana PA, van Even P, Gardi C, Lungarella G
Author(s): Minguell JJ, Erices A, Conget P
Author(s): Nakayama K
Author(s): Neuringer IP , Randell SH
Author(s): Russell RE, Culpitt SV, DeMatos C, Donnelly L, Smith M, et al.
Author(s): Lieberman J, Winter B, Sastre A
Author(s): Popescu LM, Faussone-Pellegrini MS
Author(s): Popescu LM, Manole CG, Gherghiceanu M, Ardelean A, Nicolescu MI, et al.
Author(s): Popescu LM, Manole E, Serboiu CS, Manole CG, Suciu LC, et al.
Author(s): Mandache E, Gherghiceanu M, Macarie C, Kostin S, Popescu LM
Author(s): Gherghiceanu M, Popescu LM
Author(s): Cantarero I, Luesma MJ, Junquera C
Author(s): Cantarero Carmona I, Luesma Bartolomé MJ, Junquera Escribano C
Author(s): Zheng Y, Cretoiu D, Yan G, Cretoiu SM, Popescu LM, et al.
Author(s): Galiger C, Kostin S, Golec A, Ahlbrecht K, Becker S, et al.
Author(s): Zheng Y , Cretoiu D, Yan G, Cretoiu SM, Popescu LM, et al.
Author(s): Zheng Y Bai C, Wang X
Author(s): Popescu LM, Gherghiceanu M, Suciu LC, Manole CG, Hinescu ME
Author(s): Zheng Y , Li H, Manole CG, Sun A, Ge J, et al.
Author(s): Popescu LM, Gherghiceanu M, Suciu LC, Manole CG, Hinescu ME
Author(s): Hinescu ME, Gherghiceanu M, Suciu L, Popescu LM
Author(s): Suciu LC, Popescu BO, Kostin S, Popescu LM
Author(s): Díaz-Flores L, Gutiérrez R, García MP, Sáez FJ, Díaz-Flores L Jr, et al.
Author(s): Bani D, Formigli L, Gherghiceanu M, Faussone-Pellegrini MS
Author(s): Faussone-Pellegrini MS, Bani D
Author(s): Luesma MJ, Gherghiceanu M, Popescu LM
Author(s): Ceafalan L, Gherghiceanu M, Popescu LM, Simionescu O
Author(s): Manole C G, Cisma ÅŸiu V, Gherghiceanu M, Popescu LM