Author(s): Fu LH, Wang XF, Eyal Y, She YM, Donald LJ, et al.
Selenoproteins that contain the rare amino acid selenocysteine in their primary structure have been identified in diverse organisms such as viruses, bacteria, archea, and mammals, but so far not in yeast or plants. Among the most thoroughly investigated families of selenoenzymes are the animal glutathione peroxidases (GPXs). In the last few years, genes encoding GPX-like homologues fromChlamydomonas and higher plants have been isolated, but, unlike the animal ones, all of them have cysteine (rather than selenocysteine) residues in their catalytic site. In all organisms investigated that contain selenoproteins, selenocysteine is encoded by a UGA opal codon, which is usually a stop codon. We report here that, in Chlamydomonas reinhardtii, the cDNA-cloned sequence of a GPX homologue contains an internal TGA codon in frame to the ATG. Specific mRNA expression, protein production, and enzyme activity are selenium-dependent. Sequence analysis of the peptides produced by proteolytic digestion, performed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), confirmed the presence of a selenocysteine residue at the predicted site and suggest its location in the mitochondria. Thus, our data present the first direct proof that a UGA opal codon is decoded in the plant kingdom to incorporate selenocysteine.
Referred From: http://www.jbc.org/content/277/29/25983.long
Author(s): Quinn J
Author(s): Davis DW, Oelke EA, Oplinger ES, Doll JD, Hanson CV, et al.
Author(s): Ehlers JD, Hall AE
Author(s): Martins LMV, Xavier GR, Rangel FW, Ribeiro JRA, Neves MCP, et al.
Author(s): Jayathilake C, Visvanathan R, Deen A, Bangamuwage R, Jayawardana BC, et al.
Author(s): Makela MP, Jokinen K, Kontturi M, Peltonen-Sainio M, Pehu E, et al.
Author(s): Barth C, Moeder W, Klessig DF, Conklin PL
Author(s): Barth C, De Tullio M, Conklin PL
Author(s): Domokos-Szabolcsy E
Author(s): Garg OP, Kapoor V
Author(s): Mukherjee SP, Choudhuri MA
Author(s): Gonzalez-Reyes JA, Hidalgo A, Caler JA, Palos R, Navas P
Author(s): Bartoli CG, Yu J, Gomez F, Fernandez L, Mcintosh L, et al.
Author(s): Ejaz B, Sajid ZA, Aftab F
Author(s): Niemetz R, Gross GG
Author(s): Locatelli C, Leal PC, Yunes RA, Nunes RJ, Creczynski-PasaTB
Author(s): Dai J, Mumper RJ
Author(s): Ozfidan-Konakci C, Yildiztugay E, Kucukoduk M
Author(s): Zhu YG, Pilon-Smits EAH, Zhao FJ, Williams PN, Meharg AA
Author(s): Sors TG, Ellis DR, Na GN, Lahner B, Lee S, et al.
Author(s): Huang B, Zhang J, Hou J, Chen C
Author(s): Domokos-Szabolcsy E, Marton L, Sztrik A, Babka B, Prokisch J, et al.
Author(s): Zhang J, Wang X, Xu T
Author(s): Pignocchi C, Foyer CH
Author(s): Darvishan M, Tohidi-Moghadam HR, Zahedi H
Author(s): Dolatabadian A, Sanavy SAMM, Chashmi NA (2008)
Author(s): Barh D, Srivastava HC, Mazumdar BC
Author(s): Yun X, Zijian W, Wenhua W, Peng An
Author(s): Chu J, Xiaoqin Y, Zhang Z
Author(s): Haghighi M, Abolghasemi R, Teixeira da Silva JA
Author(s): Bergmeyer HU
Author(s): Lowry OH, Rosebrough NJ, Farr AL, Randell RJ
Author(s): Umbriet WW, Burris RH, Stauffer JF
Author(s): Hartikainen H, Xue T, Piironen V
Author(s): Garcia-Bañuelos LM, Hermosillo-Cerec MA, Sanchez E
Author(s): Malik JA, Kumar S, Thakur P, Sharma S, Kaur R, et al.
Author(s): Razaji A, Asli DE, Farzanian M (2012)
Author(s): Jan SA, Bibi N, Shinwari ZK, Rabbani MA, Ullah S, et al.
Author(s): Ahmed I, Basra SMA, Wahid A
Author(s): Naz H, Akram NA, Ashraf M
Author(s): Lu CM, Zhang CY, Wu JQ, Tao MX
Author(s): Fujikura Y, Karssen CM
Author(s): Farooq M, Basra SMA, Wahid A, Khaliq A, Kobayashi N
Author(s): Abdul Khaliq, Aslam F, Matloob A, Hussain S, Geng M, et al.
Author(s): Maiti BR, Sengupta S
Author(s): Khan TA, Mazid M, Mohammad F
Author(s): Terzi R, Kalaycıoglu E, Demiralay M, Saglam A, Kadioglu A
Author(s): Patel RG, Mankad AU
Author(s): Shu K, Liu XD, Xie Q, He ZH
Author(s): Li C, Li J, Chong K, Harter K, Lee Y, et al.
Author(s): Morton LW, Abu-Amsha CR, Puddey IB, Croft KD
Author(s): Mozafariyan M, Kamelmanesh MM, Hawrylak-Nowak B
Author(s): Djanaguiraman M, Devi DD, Shanker AK, Sheeba A, Bangarusamy U
Author(s): Hawrylak-Nowak B, Matraszek R, Pogorzelec M
Author(s): FrÄ czek A, Pasterniak K
Author(s): Smrkolj P, Germ M, Kreft I, Stibilj V
Author(s): Bailly C, Benamar A, Corbineau F, Come D
Author(s): Bailly C
Author(s): Germ M, Stibilj V, Kreft I
Author(s): Niu J, Zhao L, Fan Y, Shi S, He L, et al.
Author(s): Munir N, Naz S, Aslam F, Shahzadi K, Javad S
Author(s): Malerba M, Cerana R
Author(s): Wang JD, Wang X, Wong JS