Author(s): Yan Y, Luo K, Zhang H, Chai W
Background/aims: Non-small cell lung cancer (NSCLC) constitutes around 85% of lung cancer cases and is frequently beyond surgical intervention.
Methodology: Secretory clusterin (sCLU) is found in diverse types of human cancers and is unregulated in a variety of cell lines in response to stress, and enhances cancer cell survival. However, the roles of sCLU in NSCLC are still to be elucidated.
Results: Here we show that RNA interference (RNAi)-mediated sCLU gene silencing with short interference RNA (siRNA) strongly decreased the sCLU mRNA and protein levels, as well as suppressed cell proliferation and induced cell apoptosis. In addition, sCLU siRNA also blocked the PI3K/AKT signaling pathway, and decreased the AKT phosphorylation level, but no change was found in total AKT level. More importantly, PI3K/AKT signaling pathway inhibitor, LY294002, also reduced tumor cell proliferation, which is similar to the result with or without sCLU siRNA treatment.
Conclusions: These results suggest that sCLU plays a positive role in NSCLC cell proliferation, which may be mediated by the PI3K/AKT signaling pathway. Our work in this study demonstrates RNAi-mediated sCLU gene silencing may provide a novel therapeutic strategy in the treatment of NSCLC.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/22975582
Author(s): Neal JW, Gubens MA, Wakelee HA
Author(s): Chang A
Author(s): Kartalou M, Essigmann JM
Author(s): Rabik CA, Dolan ME
Author(s): Siddik ZH
Author(s): Rosenberg ME, Silkensen J
Author(s): Niu ZH, Wang Y, Chun B, Li CX, Wu L
Author(s): Zoubeidi A, Chi K, Gleave M
Author(s): Yang GF, Li XM, Xie D
Author(s): He LR, Liu MZ, Li BK, Rao HL, Liao YJ, et al.
Author(s): Xie MJ, Motoo Y, Su SB, Mouri H, Ohtsubo K, et al.
Author(s): Park DC, Yeo SG, Wilson MR, Yerbury JJ, Kwong J, et al.
Author(s): Wang Y, Wang X, Zhao H, Liang B, Du Q
Author(s): Chou TY, Chen WC, Lee AC, Hung SM, Shih NY, et al.
Author(s): Cheng CY, Cherng SH, Wu WJ, Yang TY, Huang XY, et al.
Author(s): Laskin JJ, Nicholas G, Lee C, Gitlitz B, Vincent M, et al.
Author(s): Cao C, Shinohara ET, Li H, Niermann KJ, Kim KW, et al.
Author(s): Bortul R, Tazzari PL, Billi AM, Tabellini G, Mantovani I, et al.
Author(s): Liu D, Zhang Y, Dang C, Ma Q, Lee W, et al.
Author(s): Guinea Viniegra J, HernÃ¡ndez Losa J, SÃ¡nchez-ArÃ©valo VJ, ParadaCobo C, FernÃ¡ndezSoria VM, et al.
Author(s): McCubrey JA, Steelman LS, Abrams SL, Lee JT, Chang F, et al.
Author(s): McCubrey JA, Steelman LS, Chappell WH, Abrams SL, Wong EW, et al.
Author(s): Sridhar SS, Hedley D, Siu LL
Author(s): Tang Y, Liu F, Zheng C, Sun S, Jiang Y
Author(s): Si H, Peng C, Li J, Wang X, Zhai L, et al.
Author(s): Zhou L, Luan H, Liu Q, Jiang T, Liang H, et al.
Author(s): Li H, Schmid-Bindert G, Wang D, Zhao Y, Yang X, et al.
Author(s): Lee HY, Oh SH, Suh YA, Baek JH, Papadimitrakopoulou V, et al.
Author(s): Wang M, Liu ZM, Li XC, Yao YT, Yin ZX
Author(s): Li J, Jia L, Zhao P, Jiang Y, Zhong S, et al.
Author(s): Xiu P, Dong X, Dong X, Xu Z, Zhu H, et al.
Author(s): Ma X, Bai Y
Author(s): Scaltriti M, Bettuzzi S, Sharrard RM, Caporali A, Caccamo AE, et al.
Author(s): Manic S, Gatti L, Carenini N, Fumagalli G, Zunino F, et al.
Author(s): Springate CM, Jackson JK, Gleave ME, Burt HM
Author(s): Miyake H, Hara S, Zellweger T, Kamidono S, Gleave ME, et al.
Author(s): Hoeller C, Pratscher B, Thallinger C, Winter D, Fink D, et al.
Author(s): Redondo M, TÃ©llez T, Roldan MJ, Serrano A, GarcÃa-Aranda M, et al.
Author(s): Lourda M, Trougakos IP, Gonos ES
Author(s): Karaca B, Atmaca H, Bozkurt E, Kisim A, Uzunoglu S, et al.
Author(s): Pajak B, Orzechowski A
Author(s): Peyssonnaux C, EychÃ¨ne A
Author(s): Zhuang S, Schnellmann RG
Author(s): Ko JC, Tsai MS, Chiu YF, Weng SH, Kuo YH, et al.
Author(s): Gozdz A, Habas A, Jaworski J, Zielinska M, Albrecht J, et al.
Author(s): Huang WC, Hung MC
Author(s): Seo JH, Jeong ES, Lee KS, Heo SH, Jeong DG, et al.